Next-generation sequencing

Sequencing

Bioarray offers the most comprehensive variety of available services and technologies for sequencing of DNA, intended not only for clinical diagnosis but also as part of research projects undertaken by research centers and biotech companies.

Next-generation Sequencing Services

Bioarray takes the best of the available NGS platforms: Thermo Fisher Scientific’s Ion PGM System and Ion S5™ System, optionally automated by Ion Chef™. And Illumina’s NextSeq 500™.

These platforms offer different advantages based on their sequencing chemistries, capabilities to obtain great number of reads per run and versatility for required sample throughput.

Biorray’s sequencing services using such platforms include:

– Target sequencing: our services include the possibility of designing on-demand panels for client, typically using AmpliSeq™ technology. Also great number of panels are readily available.

– Exome Sequencing: sequencing of all coding regions of the genome. Bioarray’s exome sequencing services are based on the most efficient available capture kits and run on NextSeq 500™.

– RNA Sequencing: RNA-seq is a key tool for analysis and research of transcriptome, based on next-generation sequencing of RNA and miRNA. This provides gene expression profiling of analyzed organisms.

– Chip-seq: special technique used for identification of regions of DNA-protein interaction.

– Microbial sequencing: Bioarray offers de novo and reference-guided assembly, as well as multilocus sequence typing (MLST), for identification of microbial strains.

Capillary Sequencing services (Sanger Sequencing)

Bioarray also has an ABI-3130™ 4 capillary DNA sequencer. This is used for small scale sequencing services or genotyping.

This technology may present some advantages for particular situations:

- Longer sequencing reads.

- More stability and smaller range of errors.

- Allows validation of NGS results in the clinical context.

Plus capillary sequencing is used for specific methodologies: fragment analysis, QF-PCR assays, paternity testing, informativity testing, etc.